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DGK-α Polyclonal Antibody

E20-713831DatasheetCOAMSDS
Human;Rat;Mouse;
WB;IHC-p;IF(paraffin section);ELISA
Polyclonal, Rabbit,IgG
Price on request
Quantity

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

Gene NameDGKA

Product Information

Protein NameDiacylglycerol kinase alpha
Human Gene Id1606
Human Swiss Prot NoP23743
Mouse Swiss Prot NoO88673
SpecificityDGK-α Polyclonal Antibody detects endogenous levels of DGK-α protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameDGKA; DAGK; DAGK1; Diacylglycerol kinase alpha; DAG kinase alpha; 80 kDa diacylglycerol kinase; Diglyceride kinase alpha; DGK-alpha
Molecular Weight82672Da
Observed Band(KD)80
Functioncatalytic activity:ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.,enzyme regulation:Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C.,function:Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.,similarity:Belongs to the eukaryotic diacylglycerol kinase family.,similarity:Contains 1 DAGKc domain.,similarity:Contains 2 EF-hand domains.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,subunit:Monomer.,tissue specificity:Lymphocytes and oligodendroglial cells.,
Subcellular LocationCytoplasm, cytosol .
ExpressionExpressed in lymphocytes.
ImmunogenThe antiserum was produced against synthesized peptide derived from human DGKA. AA range:304-353
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
BackgroundThe protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008],
Western Blot analysis of various cells using 594 Secondary DGK-α Polyclonal Antibody. 50min).
Western Blot analysis of various cells using 594 Secondary DGK-α Polyclonal Antibody. 50min).
analysis of MCF7 cell. 1,primary Antibody was 1:100 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - AFluor antibody was diluted at 1:500 (room temperature,
analysis of MCF7 cell. 1,primary Antibody was 1:100 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - AFluor antibody was diluted at 1:500 (room temperature,
Western blot analysis of lysates from Jurkat Antibody was cells, using DGKA Antibody. The lane on the used for right is blocked with the synthesized peptide. (room
Western blot analysis of lysates from Jurkat Antibody was cells, using DGKA Antibody. The lane on the used for right is blocked with the synthesized peptide. (room
analysis of paraffin-embedded human tonsil. 1, diluted at 1:200 (4℃ overnight). 2, Tris-EDTA, pH9.0 was antigen retrieval. 3,Secondary antibody was diluted at 1:200 temperature, 30min).
analysis of paraffin-embedded human tonsil. 1, diluted at 1:200 (4℃ overnight). 2, Tris-EDTA, pH9.0 was antigen retrieval. 3,Secondary antibody was diluted at 1:200 temperature, 30min).
📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E20-713831-Conjugate. Price: $485 / Size: 100μl.

E20-713831-FITC$485 / 100μlE20-713831-Biotin$485 / 100μlE20-713831-HRP$485 / 100μlE20-713831-AP$485 / 100μlE20-713831-AF350$485 / 100μlE20-713831-AF405M$485 / 100μlE20-713831-AF488$485 / 100μlE20-713831-AF514$485 / 100μlE20-713831-AF532$485 / 100μlE20-713831-AF555$485 / 100μlE20-713831-AF568$485 / 100μlE20-713831-AF405S$485 / 100μlE20-713831-AF405L$485 / 100μlE20-713831-AF546$485 / 100μlE20-713831-AF594$485 / 100μlE20-713831-AF610$485 / 100μlE20-713831-AF635$485 / 100μlE20-713831-AF647$485 / 100μlE20-713831-AF680$485 / 100μlE20-713831-AF700$485 / 100μlE20-713831-AF750$485 / 100μlE20-713831-AF790$485 / 100μlE20-713831-APC$485 / 100μlE20-713831-PE$485 / 100μlE20-713831-Cy3$485 / 100μlE20-713831-Cy5$485 / 100μlE20-713831-Cy5.5$485 / 100μlE20-713831-Cy7$485 / 100μlE20-713831-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.

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