World supplier of antibodies, antigens and kits since 2007
Cell Signal Transduction Research Antibodies
DNA pol ι Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight80346DaObserved Band:85KD
Protein NameDNA polymerase iota
Human Gene Id11201
Human Swiss Prot NoQ9UNA4
Mouse Swiss Prot NoQ6R3M4
SpecificityDNA pol ι Polyclonal Antibody detects endogenous levels of DNA pol ι protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NamePOLI; RAD30B; DNA polymerase iota; Eta2; RAD30 homolog B
Functioncatalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,cofactor:Magnesium.,domain:The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.,function:Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a th
Subcellular Locationintracellular,nucleus,nucleoplasm,intracellular membrane-bounded organelle,
ExpressionBrain,Umbilical vein endothelial cell,Uterus,
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from human POLI. AA range:641-690
Gene NamePOLI
DilutionWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Backgroundcatalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,cofactor:Magnesium.,domain:The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.,function:Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.,similarity:Belongs to the DNA polymerase type-Y family.,similarity:Contains 1 umuC domain.,subcellular location:Accumulates at replication forks after DNA damage.,subunit:Binds REV1L (By similarity). Binds POLH.,tissue specificity:Ubiquitous. Highly expressed in testis.,
Conjugated Antibody
This product is also available conjugated. Format: E20-71377-Conjugate. Price: $485 / Size: 100μl.
E20-71377-FITC$485 / 100μlE20-71377-Biotin$485 / 100μlE20-71377-HRP$485 / 100μlE20-71377-AP$485 / 100μlE20-71377-AF350$485 / 100μlE20-71377-AF405M$485 / 100μlE20-71377-AF488$485 / 100μlE20-71377-AF514$485 / 100μlE20-71377-AF532$485 / 100μlE20-71377-AF555$485 / 100μlE20-71377-AF568$485 / 100μlE20-71377-AF405S$485 / 100μlE20-71377-AF405L$485 / 100μlE20-71377-AF546$485 / 100μlE20-71377-AF594$485 / 100μlE20-71377-AF610$485 / 100μlE20-71377-AF635$485 / 100μlE20-71377-AF647$485 / 100μlE20-71377-AF680$485 / 100μlE20-71377-AF700$485 / 100μlE20-71377-AF750$485 / 100μlE20-71377-AF790$485 / 100μlE20-71377-APC$485 / 100μlE20-71377-PE$485 / 100μlE20-71377-Cy3$485 / 100μlE20-71377-Cy5$485 / 100μlE20-71377-Cy5.5$485 / 100μlE20-71377-Cy7$485 / 100μlE20-71377-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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