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Cell Signal Transduction Research Antibodies
DNA pol θ Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight197597Da
Protein NameDNA polymerase theta
Human Gene Id10721
Human Swiss Prot NoO75417
SpecificityDNA pol θ Polyclonal Antibody detects endogenous levels of DNA pol θ protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NamePOLQ; POLH; DNA polymerase theta; DNA polymerase eta
Subcellular Locationnucleoplasm,chromosome,
ExpressionAorta endothelial cell,Fetal kidney,Liver,
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from human POLQ. AA range:181-230
Gene NamePOLQ
DilutionImmunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
BackgroundPOLQ (Polymerase (DNA) Theta) is a Protein Coding gene. Among its related pathways are Platinum Pathway, Pharmacokinetics/Pharmacodynamics and DNA Double-Strand Break Repair. GO annotations related to this gene include nucleic acid binding and damaged DNA binding. An important paralog of this gene is SNRNP200. NA polymerase that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA (PubMed: 25642963, PubMed: 25643323). MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation (PubMed: 25642963, PubMed: 25643323). POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends (PubMed: 25642963). POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway, thereby preventing genomic havoc by resolving unrepaired lesions (By similarity). The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ (PubMed: 25643323). The polymerase activity is highly promiscuous: unlike most polymerases, promotes extension of ssDNA and partial ssDNA (pssDNA) substrates (PubMed: 18503084, PubMed: 21050863, PubMed: 22135286). Also exhibits low-fidelity DNA synthesis, translesion synthesis and lyase activity, and it is implicated in interstrand-cross-link repair, base excision repair and DNA end-joining (PubMed: 14576298, PubMed: 18503084, PubMed: 19188258, PubMed: 24648516). Involved in somatic hypermutation of immunoglobulin genes, a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs (By similarity).
Conjugated Antibody
This product is also available conjugated. Format: E20-71376-Conjugate. Price: $485 / Size: 100μl.
E20-71376-FITC$485 / 100μlE20-71376-Biotin$485 / 100μlE20-71376-HRP$485 / 100μlE20-71376-AP$485 / 100μlE20-71376-AF350$485 / 100μlE20-71376-AF405M$485 / 100μlE20-71376-AF488$485 / 100μlE20-71376-AF514$485 / 100μlE20-71376-AF532$485 / 100μlE20-71376-AF555$485 / 100μlE20-71376-AF568$485 / 100μlE20-71376-AF405S$485 / 100μlE20-71376-AF405L$485 / 100μlE20-71376-AF546$485 / 100μlE20-71376-AF594$485 / 100μlE20-71376-AF610$485 / 100μlE20-71376-AF635$485 / 100μlE20-71376-AF647$485 / 100μlE20-71376-AF680$485 / 100μlE20-71376-AF700$485 / 100μlE20-71376-AF750$485 / 100μlE20-71376-AF790$485 / 100μlE20-71376-APC$485 / 100μlE20-71376-PE$485 / 100μlE20-71376-Cy3$485 / 100μlE20-71376-Cy5$485 / 100μlE20-71376-Cy5.5$485 / 100μlE20-71376-Cy7$485 / 100μlE20-71376-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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