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DNA Ligase IV Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-71366

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human DNA Ligase 4. AA range:616-665

  • Source:

    Polyclonal, Rabbit

  • Gene Name:

    LIG4

  • Dilution:

    WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

  • Immunohistochemical analysis of paraffin-embedded Human prostate cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

  • Background:

    DNA ligase 4(LIG4) Homo sapiens The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008],

  • Protein Name:

    DNA ligase 4

  • Human Gene Id:

    3981

  • Human Swiss Prot No:

    P49917

  • Mouse Swiss Prot No:

    Q8BTF7

  • Specificity:

    DNA Ligase IV Polyclonal Antibody detects endogenous levels of DNA Ligase IV protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    LIG4; DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4

  • MolecularWeight(Da):

    103971

  • Observed Band(KD):

    133

  • Function:

    catalytic activity:ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).,cofactor:Magnesium.,disease:Defects in LIG4 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive with sensitivity to ionizing radiation (RSSCID) [MIM:602450]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Individuals affected by RS-SCID show defects in the DNA repair machinery necessary for coding joint

  • Subcellular Location:

    nuclear chromosome, telomeric region,condensed chromosome,nucleus,nucleoplasm,cytoplasm,plasma membrane,focal adhesion,DNA-dependent protein kinase-DNA ligase 4 complex,DNA ligase IV complex,nonhomologous end joining complex,

  • Expression:

    Brain,Prostate,

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Catalog Number

  • Amount:100ul
  • Price:225$

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