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DGK-β Polyclonal Antibody
Datasheet
Catalog Number:
E20-71332Amount:
100ulReactivity:
Human,Mouse,RatPurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen..Immunogen:
The antiserum was produced against synthesized peptide derived from human DGKB. AA range:657-706Source:
Polyclonal, Rabbit
- Western Blot analysis of HuvEC cells using DGK-β Polyclonal Antibody
Gene Name:
DGKBDilution:
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.Protein Name:
Diacylglycerol kinase betaHuman Gene Id:
1607Human Swiss Prot No:
Q9Y6T7Specificity:
DGK-β Polyclonal Antibody detects endogenous levels of DGK-β protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
DGKB; DAGK2; KIAA0718; Diacylglycerol kinase beta; DAG kinase beta; 90 kDa diacylglycerol kinase; Diglyceride kinase beta; DGK-betaMolecularWeight: 90595DaObserved Band(KD):
90Background:
diacylglycerol kinase beta(DGKB) Homo sapiens Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Two alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008],Function:
catalytic activity:ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.,enzyme regulation:Stimulated by phosphatidylserine.,function:Exhibits high phosphorylation activity for long-chain diacylglycerols.,similarity:Belongs to the eukaryotic diacylglycerol kinase family.,similarity:Contains 1 DAGKc domain.,similarity:Contains 2 EF-hand domains.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,Subcellular Location:
intracellular,cytoplasm,cytosol,plasma membrane,
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