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Cytokeratin 20 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-71270

  • Amount:

    100ul

  • Molecular Weight:

    48487Da

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Keratin 20. AA range:375-424

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Cytokeratin 20 Polyclonal Antibody diluted at 1:500

  • Gene Name:

    KRT20

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

  • Protein Name:

    Keratin type I cytoskeletal 20

  • Human Gene Id:

    54474

  • Human Swiss Prot No:

    P35900

  • Mouse Gene Id:

    66809

  • Mouse Swiss Prot No:

    Q9D312

  • Rat Gene Id:

    286912

  • Rat Swiss Prot No:

    P25030

  • Specificity:

    Cytokeratin 20 Polyclonal Antibody detects endogenous levels of Cytokeratin 20 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    KRT20; Keratin; type I cytoskeletal 20; Cytokeratin-20; CK-20; Keratin-20; K20; Protein IT

  • Observed Band(KD):

    49

  • Background:

    The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008],

  • Function:

    developmental stage:First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.,function:Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.,miscellaneous:There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).,PTM:Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during t

  • Subcellular Location:

    Cytoplasm .

  • Expression:

    Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.

  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Keratin 20 Antibody. The picture on the right is blocked with the synthesized peptide.

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Cytokeratin 20 Polyclonal Antibody | EnoGene Biotech Co. Ltd