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Cytochrome b5 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-71257

  • Amount:

    100ul

  • Molecular Weight:

    15330Da

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human CYB5. AA range:61-110

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Cytochrome b5 Polyclonal Antibody

  • Gene Name:

    CYB5A

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

  • Protein Name:

    Cytochrome b5

  • Human Gene Id:

    1528

  • Human Swiss Prot No:

    P00167

  • Mouse Gene Id:

    109672

  • Mouse Swiss Prot No:

    P56395

  • Rat Gene Id:

    64001

  • Rat Swiss Prot No:

    P00173

  • Specificity:

    Cytochrome b5 Polyclonal Antibody detects endogenous levels of Cytochrome b5 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CYB5A; CYB5; Cytochrome b5; Microsomal cytochrome b5 type A; MCB5

  • Observed Band(KD):

    15

  • Background:

    The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010],

  • Function:

    disease:Defects in CYB5A are the cause of type IV hereditary methemoglobinemia [MIM:250790].,function:Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.,similarity:Belongs to the cytochrome b5 family.,similarity:Contains 1 cytochrome b5 heme-binding domain.,

  • Subcellular Location:

    [Isoform 1]: Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side. Microsome membrane; Single-pass membrane protein; Cytoplasmic side.; [Isoform 2]: Cytoplasm.

  • Expression:

    Erythrocyte,Liver,Spleen,Uterus,

  • Immunofluorescence analysis of NIH/3T3 cells, using CYB5 Antibody. The picture on the right is blocked with the synthesized peptide.

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