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CYP8B1 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-71242

  • Amount:

    100ul

  • Molecular Weight:

    58078Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Cytochrome P450 8B1. AA range:371-420

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using CYP8B1 Polyclonal Antibody

  • Gene Name:

    CYP8B1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

  • Protein Name:

    7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase

  • Human Gene Id:

    1582

  • Human Swiss Prot No:

    Q9UNU6

  • Mouse Gene Id:

    13124

  • Mouse Swiss Prot No:

    O88962

  • Specificity:

    CYP8B1 Polyclonal Antibody detects endogenous levels of CYP8B1 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CYP8B1; CYP12; 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase; 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; CYPVIIIB1; Cytochrome P450 8B1; Sterol 12-alpha-hydroxylase

  • Observed Band(KD):

    58

  • Background:

    This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless. [provided by RefSeq, Jul 2008],

  • Function:

    catalytic activity:7-alpha-hydroxycholest-4-en-3-one + NADPH + O(2) = 7-alpha,12-alpha-dihydroxycholest-4-en-3-one + NADP(+) + H(2)O.,cofactor:Heme group.,function:Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha-hydroxylated C27 steroids.,similarity:Belongs to the cytochrome P450 family.,tissue specificity:Liver.,

  • Subcellular Location:

    Endoplasmic reticulum membrane ; Single-pass membrane protein . Microsome membrane ; Single-pass membrane protein .

  • Expression:

    Liver.

  • Western blot analysis of Cytochrome P450 8B1 Antibody. The lane on the right is blocked with the Cytochrome P450 8B1 peptide.

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Catalog Number

  • Amount:100ul
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CYP8B1 Polyclonal Antibody | EnoGene Biotech Co. Ltd