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CYP2J2 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-71221

  • Amount:

    100ul

  • Molecular Weight:

    57611 DaObserved Band:50 KD

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Monkey

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Cytochrome P450 2J2. AA range:231-280

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using CYP2J2 Polyclonal Antibody

  • Gene Name:

    CYP2J2

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

  • Protein Name:

    Cytochrome P450 2J2

  • Human Gene Id:

    1573

  • Human Swiss Prot No:

    P51589

  • Specificity:

    CYP2J2 Polyclonal Antibody detects endogenous levels of CYP2J2 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CYP2J2; Cytochrome P450 2J2; Arachidonic acid epoxygenase; CYPIIJ2

  • Background:

    This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is thought to be the predominant enzyme responsible for epoxidation of endogenous arachidonic acid in cardiac tissue. Multiple transcript variants have been found for this gene. [provided by RefSeq, Jan 2016],

  • Function:

    catalytic activity:RH + reduced flavoprotein + O(2) = ROH + oxidized flavoprotein + H(2)O.,cofactor:Heme group.,function:This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools.,online information:CYP2J2 alleles,similarity:Belongs to the cytochrome P450 family.,tissue specificity:Highly expressed in heart, present at lower levels in liver, ileum, jejunum, colon, and kidney.,

  • Subcellular Location:

    Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane ; Peripheral membrane protein.

  • Expression:

    Highly expressed in heart, present at lower levels in liver, kidney and skeletal muscle (at protein level).

  • Immunofluorescence analysis of HepG2 cells, using Cytochrome P450 2J2 Antibody. The picture on the right is blocked with the synthesized peptide.

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