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CYP27A1 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-71202

  • Amount:

    100ul

  • Molecular Weight:

    60235 DaObserved Band:60 KD

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Rat;Mouse;

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Cytochrome P450 27A1. AA range:101-150

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using CYP27A1 Polyclonal Antibody diluted at 1:1000

  • Gene Name:

    CYP27A1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

  • Protein Name:

    Sterol 26-hydroxylase mitochondrial

  • Human Gene Id:

    1593

  • Human Swiss Prot No:

    Q02318

  • Mouse Swiss Prot No:

    Q9DBG1

  • Specificity:

    CYP27A1 Polyclonal Antibody detects endogenous levels of CYP27A1 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CYP27A1; CYP27; Sterol 26-hydroxylase; mitochondrial; 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase; Cytochrome P-450C27/25; Cytochrome P450 27; Sterol 27-hydroxylase; Vitamin D(3) 25-hydroxylase

  • Background:

    cytochrome P450 family 27 subfamily A member 1(CYP27A1) Homo sapiens This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein oxidizes cholesterol intermediates as part of the bile synthesis pathway. Since the conversion of cholesterol to bile acids is the major route for removing cholesterol from the body, this protein is important for overall cholesterol homeostasis. Mutations in this gene cause cerebrotendinous xanthomatosis, a rare autosomal recessive lipid storage disease. [provided by RefSeq, Jul 2008],

  • Function:

    catalytic activity:5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol + NADPH + O(2) = (25R)-5-beta-cholestane-3-alpha,7-alpha,12-alpha,26-tetraol + NADP(+) + H(2)O.,cofactor:Heme group.,disease:Defects in CYP27A1 are the cause of cerebrotendinous xanthomatosis (CTX) [MIM:213700]. CTX is a rare sterol storage disorder characterized clinically by progressive neurologic dysfunction, premature atherosclerosis, and cataracts.,function:Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity.,pathway:Hormone biosynthesis; cholecalciferol biosynthesis.,similarity:Belongs to the cytochrome P450 family.,

  • Subcellular Location:

    Mitochondrion inner membrane ; Peripheral membrane protein . Post-translationally targeted to mitochondria. All three of the receptor proteins in the TOM complex, TOMM70, TOMM20 and TOMM22 are required for the translocation across the mitochondrial outer membrane. After translocation into the matrix, associates with the inner membrane as a membrane extrinsic protein. .

  • Expression:

    Expressed in the neural retina and underlying retinal pigment epithelium (at protein level) (PubMed:21411718). Expressed in the gray and white matter of cerebellum (at protein level) (PubMed:28190002).

  • Western blot analysis of lysates from HeLa cells, using Cytochrome P450 27A1 Antibody. The lane on the right is blocked with the synthesized peptide.

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Catalog Number

  • Amount:100ul
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CYP27A1 Polyclonal Antibody | EnoGene Biotech Co. Ltd