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Cyclin H Polyclonal Antibody
Datasheet
Catalog Number:
E20-71182Amount:
100ulMolecular Weight:
37643 DaObserved Band:38 KDApplications:
WB;IHC-p;IF/ICC;ELISAReactivity:
Human;Mouse;RatPurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human Cyclin H. AA range:274-323Source:
Polyclonal, Rabbit,IgG
- Western Blot analysis of various cells using Cyclin H Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Gene Name:
CCNHDilution:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.Protein Name:
Cyclin-HHuman Gene Id:
902Human Swiss Prot No:
P51946Mouse Gene Id:
66671Mouse Swiss Prot No:
Q61458Rat Gene Id:
84389Rat Swiss Prot No:
Q9R1A0Specificity:
Cyclin H Polyclonal Antibody detects endogenous levels of Cyclin H protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
CCNH; Cyclin-H; MO15-associated protein; p34; p37Background:
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple tFunction:
function:Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.,similarity:Belongs to the cyclin family.,similarity:Belongs to the cyclin family. Cyclin C subfamily.,subunit:Associates primarily with CDK7 and MAT1 to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor.,Subcellular Location:
Nucleus.Expression:
Bone marrow,Brain,Embryonic brain,Epithelium,Liver,Urinary bladder,
- Immunofluorescence analysis of HeLa cells, using Cyclin H Antibody. The picture on the right is blocked with the synthesized peptide.
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