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CstF-50 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-71144

  • Amount:

    100ul

  • Molecular Weight:

    48358 DaObserved Band:48 KD

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human CSTF1. AA range:1-50

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using CstF-50 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

  • Gene Name:

    CSTF1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

  • Protein Name:

    Cleavage stimulation factor subunit 1

  • Human Gene Id:

    1477

  • Human Swiss Prot No:

    Q05048

  • Mouse Gene Id:

    67337

  • Mouse Swiss Prot No:

    Q99LC2

  • Rat Gene Id:

    311670

  • Rat Swiss Prot No:

    Q5BJQ6

  • Specificity:

    CstF-50 Polyclonal Antibody detects endogenous levels of CstF-50 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CSTF1; Cleavage stimulation factor subunit 1; CF-1 50 kDa subunit; Cleavage stimulation factor 50 kDa subunit; CSTF 50 kDa subunit; CstF-50

  • Background:

    This gene encodes one of three subunits which combine to form cleavage stimulation factor (CSTF). CSTF is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008],

  • Function:

    domain:N-terminus mediates homodimerization.,domain:The WD6 domain is required for interaction with BARD1. WD domains are responsible for interaction with CSTF3.,function:One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA.,PTM:The N-terminus is blocked.,similarity:Contains 6 WD repeats.,subunit:Homodimer. The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). Interacts directly with CSTF3. Interacts with BARD1.,

  • Subcellular Location:

    Nucleus .

  • Expression:

    Muscle,Testis,

  • Western blot analysis of lysates from HUVEC and 293 cells, using CSTF1 Antibody. The lane on the right is blocked with the synthesized peptide.

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Catalog Number

  • Amount:100ul
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CstF-50 Polyclonal Antibody | EnoGene Biotech Co. Ltd