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CRSP130 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-71120

  • Amount:

    100ul

  • Molecular Weight:

    156474 DaObserved Band:150 KD

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human MED23. AA range:1-50

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using CRSP130 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

  • Gene Name:

    MED23

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

  • Protein Name:

    Mediator of RNA polymerase II transcription subunit 23

  • Human Gene Id:

    9439

  • Human Swiss Prot No:

    Q9ULK4

  • Mouse Gene Id:

    70208

  • Mouse Swiss Prot No:

    Q80YQ2

  • Rat Swiss Prot No:

    Q5EB59

  • Specificity:

    CRSP130 Polyclonal Antibody detects endogenous levels of CRSP130 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    MED23; ARC130; CRSP3; DRIP130; KIAA1216; SUR2; Mediator of RNA polymerase II transcription subunit 23; Activator-recruited cofactor 130 kDa component; ARC130; Cofactor required for Sp1 transcriptional activation subunit 3; CRSP complex subu

  • Background:

    The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a metastasis suppressor. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2012],

  • Function:

    alternative products:Experimental confirmation may be lacking for some isoforms,function:Required for transcriptional activation subsequent to the assembly of the preinitiation complex (By similarity). Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.,similarity:Belongs to the

  • Subcellular Location:

    Nucleus .

  • Expression:

    Brain,Cervix carcinoma,Placenta,Prostate,Testis,

  • Immunofluorescence analysis of A549 cells, using MED23 Antibody. The picture on the right is blocked with the synthesized peptide.

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Catalog Number

  • Amount:100ul
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CRSP130 Polyclonal Antibody | EnoGene Biotech Co. Ltd