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Creatine Kinase M Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-71096

  • Amount:

    100ul

  • Molecular Weight:

    43101 DaObserved Band:43 KD

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human M-CK. AA range:10-59

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Creatine Kinase M Polyclonal Antibody

  • Gene Name:

    CKM

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

  • Protein Name:

    Creatine kinase M-type

  • Human Gene Id:

    1158

  • Human Swiss Prot No:

    P06732

  • Mouse Gene Id:

    12715

  • Mouse Swiss Prot No:

    P07310

  • Rat Gene Id:

    24265

  • Rat Swiss Prot No:

    P00564

  • Specificity:

    Creatine Kinase M Polyclonal Antibody detects endogenous levels of Creatine Kinase M protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CKM; CKMM; Creatine kinase M-type; Creatine kinase M chain; M-CK

  • Background:

    The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008],

  • Function:

    catalytic activity:ATP + creatine = ADP + phosphocreatine.,function:Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.,online information:CKM entry,online information:Creatine kinase entry,similarity:Belongs to the ATP:guanido phosphotransferase family.,subunit:Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.,

  • Subcellular Location:

    Cytoplasm.

  • Expression:

    Liver,

  • Western Blot analysis of Jurkat cells using Creatine Kinase M Polyclonal Antibody

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Catalog Number

  • Amount:100ul
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