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Cell Signal Transduction Research Antibodies
COMT Polyclonal AntibodyAntibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight30037Da
Protein NameCatechol O-methyltransferase
Human Gene Id1312
Human Swiss Prot NoP21964
Mouse Swiss Prot NoO88587
SpecificityCOMT Polyclonal Antibody detects endogenous levels of COMT protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameCOMT; Catechol O-methyltransferase
Observed Band(KD)30
Functioncatalytic activity:S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.,cofactor:Binds 1 magnesium ion per subunit.,function:Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.,mass spectrometry: PubMed:8020475,online information:Catechol-O-methyl transferase entry,polymorphism:Low enzyme activity alleles are associated with genetic susceptibility to alcoholism [MIM:103780].,polymorphism:Two alleles, COMT*1 or COMT*H with Val-158 and COMT*2 or COMT*L with Met-158 are responsible for a three to four-fold difference in enzymatic activity.,PTM:The N-terminus is blocked.,similarity:Belongs to the mammalian catechol-O-methyltransferase family.,tissue specificity:Brain, liver, placenta,
Subcellular Locationmitochondrion,cytosol,plasma membrane,membrane,integral component of membrane,axon,dendritic spine,cell body,postsynaptic membrane,extracellular exosome,
ExpressionBrain,Muscle,Placenta,Skin,
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from human COMT. AA range:61-110
Gene NameCOMT
DilutionWestern Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Backgroundcatechol-O-methyltransferase(COMT) Homo sapiens Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008],
Conjugated Antibody
This product is also available conjugated. Format: E20-71040-Conjugate. Price: $485 / Size: 100μl.
E20-71040-FITC$485 / 100μlE20-71040-Biotin$485 / 100μlE20-71040-HRP$485 / 100μlE20-71040-AP$485 / 100μlE20-71040-AF350$485 / 100μlE20-71040-AF405M$485 / 100μlE20-71040-AF488$485 / 100μlE20-71040-AF514$485 / 100μlE20-71040-AF532$485 / 100μlE20-71040-AF555$485 / 100μlE20-71040-AF568$485 / 100μlE20-71040-AF405S$485 / 100μlE20-71040-AF405L$485 / 100μlE20-71040-AF546$485 / 100μlE20-71040-AF594$485 / 100μlE20-71040-AF610$485 / 100μlE20-71040-AF635$485 / 100μlE20-71040-AF647$485 / 100μlE20-71040-AF680$485 / 100μlE20-71040-AF700$485 / 100μlE20-71040-AF750$485 / 100μlE20-71040-AF790$485 / 100μlE20-71040-APC$485 / 100μlE20-71040-PE$485 / 100μlE20-71040-Cy3$485 / 100μlE20-71040-Cy5$485 / 100μlE20-71040-Cy5.5$485 / 100μlE20-71040-Cy7$485 / 100μlE20-71040-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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