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Claudin-5 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-70953

  • Amount:

    100ul

  • Molecular Weight:

    23147Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Claudin 5. AA range:169-218

  • Source:

    Polyclonal, Rabbit,IgG

  • Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.

  • Gene Name:

    CLDN5

  • Dilution:

    Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.

  • Protein Name:

    Claudin-5

  • Human Gene Id:

    7122

  • Human Swiss Prot No:

    O00501

  • Mouse Gene Id:

    12741

  • Mouse Swiss Prot No:

    O54942

  • Rat Gene Id:

    65131

  • Rat Swiss Prot No:

    Q9JKD6

  • Specificity:

    Claudin-5 Polyclonal Antibody detects endogenous levels of Claudin-5 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CLDN5; AWAL; TMVCF; Claudin-5; Transmembrane protein deleted in VCFS; TMDVCF

  • Observed Band(KD):

    23

  • Background:

    This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2008],

  • Function:

    function:Plays a major role in tight junction-specific obliteration of the intercellular space.,similarity:Belongs to the claudin family.,subunit:Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with MPDZ.,

  • Subcellular Location:

    Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.

  • Expression:

    Adipose tissue,Brain,Lung,Prostate,Products Images

  • Immunofluorescence analysis of human-liver tissue. 1,Claudin-5 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

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Catalog Number

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