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ChoKB Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-70910

  • Amount:

    100ul

  • Molecular Weight:

    45271Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human CHKB. AA range:301-350

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using ChoKB Polyclonal Antibody

  • Gene Name:

    CHKB

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

  • Protein Name:

    Choline/ethanolamine kinase

  • Human Gene Id:

    1120

  • Human Swiss Prot No:

    Q9Y259

  • Mouse Swiss Prot No:

    O55229

  • Specificity:

    ChoKB Polyclonal Antibody detects endogenous levels of ChoKB protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CHKB; CHETK; CHKL; Choline/ethanolamine kinase; Choline kinase beta; CK; CKB; Choline kinase-like protein; Ethanolamine kinase; EK; Ethanolamine kinase beta; EKB; choline/ethanolamine kinase beta; CKEKB

  • Observed Band(KD):

    45

  • Background:

    Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq, Jun 2009],

  • Function:

    catalytic activity:ATP + choline = ADP + O-phosphocholine.,catalytic activity:ATP + ethanolamine = ADP + O-phosphoethanolamine.,catalytic activity:Palmitoyl-CoA + L-carnitine = CoA + L-palmitoylcarnitine.,miscellaneous:This protein is produced by a bicistronic gene which also produces the CHKB protein from a non-overlapping reading frame.,miscellaneous:This protein is produced by a bicistronic gene which also produces the CPT1B protein from a non-overlapping reading frame.,pathway:Lipid metabolism; fatty acid beta-oxidation.,similarity:Belongs to the carnitine/choline acetyltransferase family.,similarity:Belongs to the choline/ethanolamine kinase family.,tissue specificity:Strong expression in heart and skeletal muscle. No expression in liver and kidney.,

  • Subcellular Location:

    cytosol,

  • Expression:

    Brain,Clones donated by RIKEN,Epithelium,Heart,Human lung,Lung,Skeletal muscle,T-ceProducts Images

  • Western blot analysis of lysates from HepG2 and A549 cells, using CHKB Antibody. The lane on the right is blocked with the synthesized peptide.

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Catalog Number

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ChoKB Polyclonal Antibody | EnoGene Biotech Co. Ltd