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Cell Signal Transduction Research Antibodies
Chk2 Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight60915DaObserved Band:61KD
Protein NameSerine/threonine-protein kinase Chk2
Human Gene Id11200
Human Swiss Prot NoO96017
Mouse Gene Id50883
Mouse Swiss Prot NoQ9Z265
SpecificityChk2 Polyclonal Antibody detects endogenous levels of Chk2 protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameCHEK2; CDS1; CHK2; RAD53; Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2
Functioncatalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,disease:Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53.,disease:Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM:259500].,disease:Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM:176807].,enzyme regulation:Rapidly phosphorylated on Thr-68 by MLTK in response to DNA damage and to replication block. Kinase activity is also up-regulated by autophosphorylation.,function:Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53
Subcellular Locationchromosome, telomeric region,nucleoplasm,Golgi apparatus,PML body,
ExpressionColon carcinoma,Mammary gland,Muscle,T-cell,
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from human CHEK2. AA range:35-84
Gene NameCHEK2
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications
Backgroundcheckpoint kinase 2(CHEK2) Homo sapiens In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutati
Conjugated Antibody
This product is also available conjugated. Format: E20-70905-Conjugate. Price: $485 / Size: 100μl.
E20-70905-FITC$485 / 100μlE20-70905-Biotin$485 / 100μlE20-70905-HRP$485 / 100μlE20-70905-AP$485 / 100μlE20-70905-AF350$485 / 100μlE20-70905-AF405M$485 / 100μlE20-70905-AF488$485 / 100μlE20-70905-AF514$485 / 100μlE20-70905-AF532$485 / 100μlE20-70905-AF555$485 / 100μlE20-70905-AF568$485 / 100μlE20-70905-AF405S$485 / 100μlE20-70905-AF405L$485 / 100μlE20-70905-AF546$485 / 100μlE20-70905-AF594$485 / 100μlE20-70905-AF610$485 / 100μlE20-70905-AF635$485 / 100μlE20-70905-AF647$485 / 100μlE20-70905-AF680$485 / 100μlE20-70905-AF700$485 / 100μlE20-70905-AF750$485 / 100μlE20-70905-AF790$485 / 100μlE20-70905-APC$485 / 100μlE20-70905-PE$485 / 100μlE20-70905-Cy3$485 / 100μlE20-70905-Cy5$485 / 100μlE20-70905-Cy5.5$485 / 100μlE20-70905-Cy7$485 / 100μlE20-70905-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
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