World supplier of antibodies, antigens and kits since 2007
Cell Signal Transduction Research Antibodies
CENP-A Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight15991 Da
Protein NameHistone H3-like centromeric protein A
Human Gene Id1058
Human Swiss Prot NoP49450
Mouse Swiss Prot NoO35216
SpecificityCENP-A Polyclonal Antibody detects endogenous levels of CENP-A protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameCENPA; Histone H3-like centromeric protein A; Centromere autoantigen A; Centromere protein A; CENP-A
Functiondisease:Antibodies against CENPA are present in sera from patients with autoimmune diseases that developed autoantibodies against centrosomal proteins.,domain:The CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containing CENPA and is necessary and sufficient to mediate the localization into centromeres.,function:Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.,PTM:Phosphorylation of Ser-7 by Aurora-A/STK6 and Aurora-B/STK12 during prophase is required for localization of Aurora-A/STK6 and Aurora-B/STK12 at inner centrome
Subcellular Locationchromosome, centromeric region,condensed nuclear chromosome kinetochore,condensed nuclear chromosome, centromeric region,nucleosome,nuclear nucleosome,condensed chromosome inner kinetochore,nucleus,nucleoplasm,cytosol,
ExpressionEpithelium,Uterus,
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from human Centromeric Protein A. AA range:1-50
Gene NameCENPA
DilutionImmunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Backgroundcentromere protein A(CENPA) Homo sapiens Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2015],
Conjugated Antibody
This product is also available conjugated. Format: E20-70853-Conjugate. Price: $485 / Size: 100μl.
E20-70853-FITC$485 / 100μlE20-70853-Biotin$485 / 100μlE20-70853-HRP$485 / 100μlE20-70853-AP$485 / 100μlE20-70853-AF350$485 / 100μlE20-70853-AF405M$485 / 100μlE20-70853-AF488$485 / 100μlE20-70853-AF514$485 / 100μlE20-70853-AF532$485 / 100μlE20-70853-AF555$485 / 100μlE20-70853-AF568$485 / 100μlE20-70853-AF405S$485 / 100μlE20-70853-AF405L$485 / 100μlE20-70853-AF546$485 / 100μlE20-70853-AF594$485 / 100μlE20-70853-AF610$485 / 100μlE20-70853-AF635$485 / 100μlE20-70853-AF647$485 / 100μlE20-70853-AF680$485 / 100μlE20-70853-AF700$485 / 100μlE20-70853-AF750$485 / 100μlE20-70853-AF790$485 / 100μlE20-70853-APC$485 / 100μlE20-70853-PE$485 / 100μlE20-70853-Cy3$485 / 100μlE20-70853-Cy5$485 / 100μlE20-70853-Cy5.5$485 / 100μlE20-70853-Cy7$485 / 100μlE20-70853-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
Categories by research area
Categories by product type
- Antibodies
- Tag antibodies
- Internal control antibodies
- Pathological (Immunohistochemistry, IHC) antibodies
- Cell Signal Transduction Research Antibodies
- Secondary Antibody
- Antibody Sampler Kits
- Recombinant Proteins
- Fluorescence Reagents
- Signal Inhibitors and Activators
- Transfection Reagent
- Cell biology kits