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Cdk2 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-70832

  • Amount:

    100ul

  • Molecular Weight:

    33930Da

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human CDK2. AA range:231-280

  • Source:

    Polyclonal, Rabbit,IgG

  • Cen, Chaoqun, et al. "Long noncoding RNA LINC01510 promotes the growth of colorectal cancer cells by modulating MET expression." Cancer cell international 18.1 (2018): 45.

  • Gene Name:

    CDK2

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

  • Protein Name:

    Cyclin-dependent kinase 2

  • Human Gene Id:

    1017

  • Human Swiss Prot No:

    P24941

  • Mouse Gene Id:

    12566

  • Mouse Swiss Prot No:

    P97377

  • Rat Swiss Prot No:

    Q63699

  • Specificity:

    Cdk2 Polyclonal Antibody detects endogenous levels of Cdk2 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CDK2; CDKN2; Cyclin-dependent kinase 2; Cell division protein kinase 2; p33 protein kinase

  • Observed Band(KD):

    32

  • Background:

    cyclin dependent kinase 2(CDK2) Homo sapiens This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014],

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-160 activates it.,function:Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Found in a complex with CABLES1, CCNA1 and CCNE1. Interacts with CABLES1 (By similarity). Interacts with UHRF2. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts with the Speedy/Ringo proteins SPDYA and SPDYC. Found in a complex with both SPDYA and CDKN1B/KIP1.,

  • Subcellular Location:

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus, Cajal body. Cytoplasm. Endosome. Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3).

  • Expression:

    Epithelium,Lung,Placenta,

  • Immunofluorescence analysis of Hela cell. 1,Cdk2 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). NSE Monoclonal Antibody(13E2)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).

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Catalog Number

  • Amount:100ul
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