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CDC46 Polyclonal Antibody
Datasheet
Catalog Number:
E20-70812Amount:
100ulMolecular Weight:
82286DaApplications:
WB;IHC-p;IF/ICC;ELISAReactivity:
Human;Mouse;RatPurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human MCM5. AA range:21-70Source:
Polyclonal, Rabbit,IgG
- Western Blot analysis of various cells using CDC46 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Gene Name:
MCM5Dilution:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.Protein Name:
DNA replication licensing factor MCM5Human Gene Id:
4174Human Swiss Prot No:
P33992Mouse Swiss Prot No:
P49718Specificity:
CDC46 Polyclonal Antibody detects endogenous levels of CDC46 protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
MCM5; CDC46; DNA replication licensing factor MCM5; CDC46 homolog; P1-CDC46Observed Band(KD):
90Background:
The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation. [provided by RefSeq, Jul 2008],Function:
similarity:Belongs to the MCM family.,similarity:Contains 1 MCM domain.,Subcellular Location:
Nucleus . Chromosome . Cytoplasm, cytosol . Associated with chromatin before the formation of nuclei and detaches from it as DNA replication progresses. .Expression:
Cervix,Cervix carcinoma,Lymph,Placenta,Spleen,Thymus,Tongue
- Western Blot analysis of HepG2 cells using CDC46 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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