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Cdc34 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-70803

  • Amount:

    100ul

  • Molecular Weight:

    26737Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from the Internal region of human CDC34. AA range:121-170

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of K562, PC-3, mouse liver cells using Cdc34 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).

  • Gene Name:

    CDC34

  • Dilution:

    Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.

  • Protein Name:

    Ubiquitin-conjugating enzyme E2 R1

  • Human Gene Id:

    997

  • Human Swiss Prot No:

    P49427

  • Mouse Gene Id:

    216150

  • Mouse Swiss Prot No:

    Q8CFI2

  • Specificity:

    Cdc34 Polyclonal Antibody detects endogenous levels of Cdc34 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CDC34; UBCH3; UBE2R1; Ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; Ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1

  • Observed Band(KD):

    34

  • Background:

    The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq, Jul 2008],

  • Function:

    catalytic activity:ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.,function:Catalyzes the covalent attachment of ubiquitin to other proteins. May be involved in degradation of katenin.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the ubiquitin-conjugating enzyme family.,subunit:Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. When phosphorylated, interacts with beta-TrCP (BTRC).,

  • Subcellular Location:

    Cytoplasm. Nucleus. The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase.

  • Expression:

    Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription.

  • Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100

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Catalog Number

  • Amount:100ul
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Cdc34 Polyclonal Antibody | EnoGene Biotech Co. Ltd