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Cdc27 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-70802

  • Amount:

    100ul

  • Molecular Weight:

    91867Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human H-NUC. AA range:361-410

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Cdc27 Polyclonal Antibody

  • Gene Name:

    CDC27

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

  • Protein Name:

    Cell division cycle protein 27 homolog

  • Human Gene Id:

    996

  • Human Swiss Prot No:

    P30260

  • Mouse Gene Id:

    217232

  • Mouse Swiss Prot No:

    A2A6Q5

  • Rat Gene Id:

    360643

  • Rat Swiss Prot No:

    Q4V8A2

  • Specificity:

    Cdc27 Polyclonal Antibody detects endogenous levels of Cdc27 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CDC27; ANAPC3; D0S1430E; D17S978E; Cell division cycle protein 27 homolog; Anaphase-promoting complex subunit 3; APC3; CDC27 homolog; CDC27Hs; H-NUC

  • Observed Band(KD):

    92

  • Background:

    The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been i

  • Function:

    PTM:Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.,similarity:Belongs to the APC3/CDC27 family.,similarity:Contains 9 TPR repeats.,subunit:Interacts with RB.,

  • Subcellular Location:

    Nucleus . Cytoplasm, cytoskeleton, spindle .

  • Expression:

    Epithelium,Uterus,

  • Western blot analysis of lysates from HUVEC, HepG2, and COLO205 cells, using H-NUC Antibody. The lane on the right is blocked with the synthesized peptide.

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Catalog Number

  • Amount:100ul
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Cdc27 Polyclonal Antibody | EnoGene Biotech Co. Ltd