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Cdc20 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-70791

  • Amount:

    100ul

  • Molecular Weight:

    54723Da

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human p55CDC. AA range:81-130

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Cdc20 Polyclonal Antibody diluted at 1:2000

  • Gene Name:

    CDC20

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

  • Protein Name:

    Cell division cycle protein 20 homolog

  • Human Gene Id:

    991

  • Human Swiss Prot No:

    Q12834

  • Mouse Gene Id:

    107995

  • Mouse Swiss Prot No:

    Q9JJ66

  • Rat Gene Id:

    64515

  • Rat Swiss Prot No:

    Q62623

  • Specificity:

    Cdc20 Polyclonal Antibody detects endogenous levels of Cdc20 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CDC20; Cell division cycle protein 20 homolog; p55CDC

  • Observed Band(KD):

    50

  • Background:

    CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. [provided by RefSeq, Jul 2008],

  • Function:

    developmental stage:Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.,function:Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated during mitosis, probably by maturation promoting factor (MPF).,PTM:Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint.,similarity:Belongs to the WD repeat CDC20/Fizzy family.,similarity:Contains 7 WD repeats.,subunit:Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with SPATC1 (By similarity). Interacts with MAD2L

  • Subcellular Location:

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole .

  • Expression:

    Colon,Colon adenocarcinoma,Liver,Lymph,Muscle,Ovary,Skin,Spleen,Testis,

  • Western Blot analysis of 3T3 cells using Cdc20 Polyclonal Antibody diluted at 1:2000

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Catalog Number

  • Amount:100ul
  • Price:225$

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Cdc20 Polyclonal Antibody | EnoGene Biotech Co. Ltd