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CD9 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-70782

  • Amount:

    100ul

  • Molecular Weight:

    25416Da

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human CD9. AA range:101-150

  • Source:

    Polyclonal, Rabbit,IgG

  • Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.

  • Gene Name:

    CD9

  • Dilution:

    Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.

  • Protein Name:

    CD9 antigen

  • Human Gene Id:

    928

  • Human Swiss Prot No:

    P21926

  • Mouse Gene Id:

    12527

  • Mouse Swiss Prot No:

    P40240

  • Rat Gene Id:

    24936

  • Rat Swiss Prot No:

    P40241

  • Specificity:

    CD9 Polyclonal Antibody detects endogenous levels of CD9 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CD9; MIC3; TSPAN29; GIG2; CD9 antigen; 5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; MRP-1; Tetraspanin-29; Tspan-29; p24; CD antigen CD9

  • Observed Band(KD):

    25

  • Background:

    This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays a critical role in the suppression of cancer cell motility and metastasis. [provided by RefSeq, Jan 2011],

  • Function:

    function:Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.,PTM:Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.,similarity:Belongs to the tetraspanin (TM4SF) family.,subunit:Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Associates with CR2/CD21 and with PTGFRN/CD9P1. Interacts directly with IGSF8.,tissue specificity:Expressed by a variety of hematopoietic and epithelial cells.,

  • Subcellular Location:

    Cell membrane ; Multi-pass membrane protein . Membrane ; Multi-pass membrane protein . Secreted, extracellular exosome . Present at the cell surface of oocytes. Accumulates in the adhesion area between the sperm and egg following interaction between IZUMO1 and its receptor IZUMO1R/JUNO. .

  • Expression:

    Detected in platelets (at protein level) (PubMed:19640571). Expressed by a variety of hematopoietic and epithelial cells (PubMed:19640571).

  • Western Blot analysis of various cells using CD9 Polyclonal Antibody diluted at 1:1000

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Catalog Number

  • Amount:100ul
  • Price:225$

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CD9 Polyclonal Antibody | EnoGene Biotech Co. Ltd