World supplier of antibodies, antigens and kits since 2007
Cell Signal Transduction Research Antibodies
CD3EAP Polyclonal Antibody
Polyclonal, Rabbit
$225/ 100ul
QuantityTotal: $225
Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.
Product Information
Molecular Weight54986Da
Protein NameDNA-directed RNA polymerase I subunit RPA34
Human Gene Id10849
Human Swiss Prot NoO15446
Mouse Swiss Prot NoQ76KJ5
SpecificityCD3EAP Polyclonal Antibody detects endogenous levels of CD3EAP protein.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration1 mg/ml
Storage Stability-20°C/1 year
Other NameCD3EAP; ASE1; CAST; PAF49; DNA-directed RNA polymerase I subunit RPA34; A34.5; Antisense to ERCC-1 protein; ASE-1; CD3-epsilon-associated protein; CAST; CD3E-associated protein; RNA polymerase I-associated factor PAF49
Observed Band(KD)55
Functioncaution:It is not known whether the so-called human ASE1 and human CAST proteins represent two sides of a single gene product with sharply different functional characteristics. Experiments done with the mouse homolog protein are in favor of an implication of this gene in rRNA transcription instead of T-cell receptor signaling.,function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.,function:Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.,miscellaneous:It is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC1. This gene overlap is conserved in mouse suggesting an
Subcellular LocationRNA polymerase I transcription factor complex,nucleus,nucleoplasm,chromosome,nucleolus,DNA-directed RNA polymerase I complex,cytoplasm,mitochondrion,
ExpressionEye,Serum,Skin,Testis,
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ImmunogenThe antiserum was produced against synthesized peptide derived from human CD3EAP. AA range:441-490
Gene NameCD3EAP
DilutionWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Backgroundcaution:It is not known whether the so-called human ASE1 and human CAST proteins represent two sides of a single gene product with sharply different functional characteristics. Experiments done with the mouse homolog protein are in favor of an implication of this gene in rRNA transcription instead of T-cell receptor signaling.,function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.,function:Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.,miscellaneous:It is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC1. This gene overlap is conserved in mouse suggesting an important biologic function.,PTM:Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes.,PTM:Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other group.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the eukaryotic RPA34 RNA polymerase subunit family.,subcellular location:Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes.,subunit:Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with TAF1A thereby associates with the SL1 complex. Interacts with UBTF. Interacts with POLR1E/PRAF1 through its N-terminal region (By similarity). Isoform 2 interacts with CD3E.,
Conjugated Antibody
This product is also available conjugated. Format: E20-70759-Conjugate. Price: $485 / Size: 100μl.
E20-70759-FITC$485 / 100μlE20-70759-Biotin$485 / 100μlE20-70759-HRP$485 / 100μlE20-70759-AP$485 / 100μlE20-70759-AF350$485 / 100μlE20-70759-AF405M$485 / 100μlE20-70759-AF488$485 / 100μlE20-70759-AF514$485 / 100μlE20-70759-AF532$485 / 100μlE20-70759-AF555$485 / 100μlE20-70759-AF568$485 / 100μlE20-70759-AF405S$485 / 100μlE20-70759-AF405L$485 / 100μlE20-70759-AF546$485 / 100μlE20-70759-AF594$485 / 100μlE20-70759-AF610$485 / 100μlE20-70759-AF635$485 / 100μlE20-70759-AF647$485 / 100μlE20-70759-AF680$485 / 100μlE20-70759-AF700$485 / 100μlE20-70759-AF750$485 / 100μlE20-70759-AF790$485 / 100μlE20-70759-APC$485 / 100μlE20-70759-PE$485 / 100μlE20-70759-Cy3$485 / 100μlE20-70759-Cy5$485 / 100μlE20-70759-Cy5.5$485 / 100μlE20-70759-Cy7$485 / 100μlE20-70759-PE-Cy5$485 / 100μl
Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.
Categories by research area
Categories by product type
- Antibodies
- Tag antibodies
- Internal control antibodies
- Pathological (Immunohistochemistry, IHC) antibodies
- Cell Signal Transduction Research Antibodies
- Secondary Antibody
- Antibody Sampler Kits
- Recombinant Proteins
- Fluorescence Reagents
- Signal Inhibitors and Activators
- Transfection Reagent
- Cell biology kits