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CBP80 Polyclonal Antibody
Datasheet
Catalog Number:
E20-70697Amount:
100ulMolecular Weight:
91839DaApplications:
WB;IHC-p;IF(paraffin section);ELISAReactivity:
Human;Mouse;RatPurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human NCBP1. AA range:1-50Source:
Polyclonal, Rabbit,IgG
- Western Blot analysis of various cells using CBP80 Polyclonal Antibody diluted at 1:500
Gene Name:
NCBP1Dilution:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.Protein Name:
Nuclear cap-binding protein subunit 1Human Gene Id:
4686Human Swiss Prot No:
Q09161Mouse Gene Id:
433702Mouse Swiss Prot No:
Q3UYV9Rat Gene Id:
298075Rat Swiss Prot No:
Q56A27Specificity:
CBP80 Polyclonal Antibody detects endogenous levels of CBP80 protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
NCBP1; CBP80; NCBP; Nuclear cap-binding protein subunit 1; 80 kDa nuclear cap-binding protein; CBP80; NCBP 80 kDa subunitObserved Band(KD):
80Background:
The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. [provided by RefSeq, Jul 2008],Function:
function:Involved in mediating U snRNA export from the nucleus. Binds to 5' capped mRNA.,similarity:Belongs to the NCBP1 family.,similarity:Contains 1 MIF4G domain.,subunit:Found in a U snRNA export complex with RNUXA/PHAX, CBP20, CBP80, RAN, XPO1 and m7G-capped RNA. Interaction with RNUXA/PHAX (By similarity). Heterodimer with NCBP2. Is part of the exon junction complex (EJC) containing NCBP1, NCBP2, RNPS1, RBM8A, SRRM1, NXF1, UPF3B, UPF2, THOC4 and/or REFBP2.,Subcellular Location:
Nucleus . Cytoplasm . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .Expression:
Brain,Epithelium,Placenta,
- Western blot analysis of lysates from HT-29 and LOVO cells, using NCBP1 Antibody. The lane on the right is blocked with the synthesized peptide.
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