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Calponin 2 Polyclonal Antibody
Datasheet
Catalog Number:
E20-70619Amount:
100ulMolecular Weight:
33697DaApplications:
WB;IF/ICC;ELISAReactivity:
Human;MousePurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human CNN2. AA range:121-170Source:
Polyclonal, Rabbit,IgG
- Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Gene Name:
CNN2Dilution:
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.Protein Name:
Calponin-2Human Gene Id:
1265Human Swiss Prot No:
Q99439Mouse Gene Id:
12798Mouse Swiss Prot No:
Q08093Specificity:
Calponin 2 Polyclonal Antibody detects endogenous levels of Calponin 2 protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
CNN2; Calponin-2; Calponin H2; smooth muscle; Neutral calponinObserved Band(KD):
33Background:
The protein encoded by this gene, which can bind actin, calmodulin, troponin C, and tropomyosin, may function in the structural organization of actin filaments. The encoded protein could play a role in smooth muscle contraction and cell adhesion. Several pseudogenes of this gene have been identified, and are present on chromosomes 1, 2, 3, 6, 9, 11, 13, 15, 16, 21 and 22. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015],Function:
function:Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.,similarity:Belongs to the calponin family.,similarity:Contains 1 CH (calponin-homology) domain.,similarity:Contains 3 calponin-like repeats.,tissue specificity:Heart and smooth muscle.,Subcellular Location:
stress fiber,cytoskeleton,cell-cell junction,cell-cell adherens junction,focal adhesion,membrane,extracellular exosome,Expression:
Heart and smooth muscle.
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