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BID Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-70488

  • Amount:

    100ul

  • Molecular Weight:

    21995DaObserved Band:25KD

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Rat;Mouse;

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human BID. AA range:44-93

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using BID Polyclonal Antibody diluted at 1:1000

  • Gene Name:

    BID

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

  • Protein Name:

    BH3-interacting domain death agonist

  • Human Gene Id:

    637

  • Human Swiss Prot No:

    P55957

  • Mouse Gene Id:

    12122

  • Mouse Swiss Prot No:

    P70444

  • Specificity:

    BID Polyclonal Antibody detects endogenous levels of BID protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    BID; BH3-interacting domain death agonist; p22 BID; BID

  • Background:

    This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008],

  • Function:

    domain:Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.,function:The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.,subcellular location:A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively.,subcellular location:Associated with the mitochondrial membrane.,subcellular location:Translocates to mitochondria as an integral membrane protein.,subcellular location:When uncleaved

  • Subcellular Location:

    Cytoplasm . Mitochondrion membrane . Mitochondrion outer membrane . When uncleaved, it is predominantly cytoplasmic. .; [BH3-interacting domain death agonist p15]: Mitochondrion membrane . Translocates to mitochondria as an integral membrane protein. .; [BH3-interacting domain death agonist p13]: Mitochondrion membrane . Associated with the mitochondrial membrane. .; [Isoform 1]: Cytoplasm .; [Isoform 3]: Cytoplasm .; [Isoform 2]: Mitochondrion membrane . A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively. .

  • Expression:

    [Isoform 2]: Expressed in spleen, pancreas and placenta (at protein level). ; [Isoform 3]: Expressed in lung, pancreas and spleen (at protein level). ; [Isoform 4]: Expressed in lung and pancreas (at protein level).

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Catalog Number

  • Amount:100ul
  • Price:225$

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BID Polyclonal Antibody | EnoGene Biotech Co. Ltd