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ATP5H Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-70406

  • Amount:

    100ul

  • Molecular Weight:

    18491DaObserved Band:26KD

  • Applications:

    WB;IHC-p;IF(paraffin section);ELISA

  • Reactivity:

    Human;Rat;Mouse;

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human ATP5H. AA range:111-160

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using ATP5H Polyclonal Antibody diluted at 1:2000

  • Gene Name:

    ATP5H

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

  • Protein Name:

    ATP synthase subunit d mitochondrial

  • Human Gene Id:

    10476

  • Human Swiss Prot No:

    O75947

  • Mouse Gene Id:

    71679

  • Mouse Swiss Prot No:

    Q9DCX2

  • Rat Gene Id:

    641434

  • Rat Swiss Prot No:

    P31399

  • Specificity:

    ATP5H Polyclonal Antibody detects endogenous levels of ATP5H protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    ATP5H; My032; ATP synthase subunit d; mitochondrial; ATPase subunit d

  • Background:

    Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq, Jun 2010],

  • Function:

    function:Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.,similarity:Belongs to the ATPase d subunit family.,subunit:F-type ATPases have 2 components, CF(1) - the

  • Subcellular Location:

    Mitochondrion. Mitochondrion inner membrane.

  • Expression:

    Bone marrow,Brain,Fetal brain,Fetal

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ATP5H Polyclonal Antibody | EnoGene Biotech Co. Ltd