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ASF1B Polyclonal Antibody
Datasheet
Catalog Number:
E20-70369Amount:
100ulPrice:
225$Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human ASF1B. AA range:101-150Source:
Polyclonal, RabbitGene Name:
ASF1BDilution:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
- Western blot analysis of lysates from K562 cells, using ASF1B Antibody. The lane on the right is blocked with the synthesized peptide.
Background:
anti-silencing function 1B histone chaperone(ASF1B) Homo sapiens This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. [provided by RefSeq, Jul 2008],Protein Name:
Histone chaperone ASF1BHuman Gene Id:
55723Human Swiss Prot No:
Q9NVP2Mouse Gene Id:
66929Mouse Swiss Prot No:
Q9DAP7Specificity:
ASF1B Polyclonal Antibody detects endogenous levels of ASF1B protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
ASF1B; Histone chaperone ASF1B; Anti-silencing function protein 1 homolog B; hAsf1; hAsf1b; CCG1-interacting factor A-II; CIA-II; hCIA-IIMolecularWeight(Da):
22434Observed Band(KD):
26Function:
function:Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis.,PTM:Phosphorylated by TLK1 and TLK2.,similarity:Belongs to the ASF1 family.,subunit:Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with HAT1, NASP, TAF1, TLK1 and TLK2.,tissue specificity:Highly expressed in testis and at lower levels in colon, small intestine and thymus.,Subcellular Location:
chromatin,nuclear chromatin,nucleus,nucleoplasm,protein complex,Expression:
Teratocarcinoma,Testis,Uterus,
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Catalog Number
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