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Asf1a Polyclonal Antibody
Datasheet
Catalog Number:
E20-70368Amount:
100ulMolecular Weight:
22969DaObserved Band:23KDApplications:
WB;IF/ICC;ELISAReactivity:
Human;Mouse;Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human ASF1A. AA range:121-170Source:
Polyclonal, Rabbit,IgG
- Western Blot analysis of various cells using Asf1a Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.
Gene Name:
ASF1ADilution:
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.Protein Name:
Histone chaperone ASF1AHuman Gene Id:
25842Human Swiss Prot No:
Q9Y294Mouse Gene Id:
66403Mouse Swiss Prot No:
Q9CQE6Specificity:
Asf1a Polyclonal Antibody detects endogenous levels of Asf1a protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
ASF1A; CGI-98; HSPC146; Histone chaperone ASF1A; Anti-silencing function protein 1 homolog A; hAsf1; hAsf1a; CCG1-interacting factor A; CIA; hCIABackground:
This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. [provided by RefSeq, Jul 2008],Function:
function:Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.,PTM:Phosphorylated by TLK1 and TLK2. Highly phosphorylated in S-phase and at lower levels in M-phase.,similarity:Belongs to the ASF1 family.,subunit:Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with CABIN1, HAT1, HIRA, NASP, TAF1, TLK1, TLK2 and UBN1.,tissue specificity:Ubiquitously expressed.,Subcellular Location:
Nucleus .Expression:
Ubiquitously expressed.
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