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ARHGAP1 Polyclonal Antibody
Datasheet
Catalog Number:
E20-70313Amount:
100ulApplications:
WB;IHC-p;IF(paraffin section);ELISAReactivity:
Human;MousePurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human RHG1. AA range:189-238Source:
Polyclonal, RabbitGene Name:
ARHGAP1Dilution:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
- Immunohistochemistry analysis of paraffin-embedded human heart tissue, using RHG1 Antibody. The picture on the right is blocked with the synthesized peptide.
Background:
Rho GTPase activating protein 1(ARHGAP1) Homo sapiens This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein contains a SRC homology 3 domain and interacts with Bcl-2-associated protein family members. [provided by RefSeq, Aug 2012],Protein Name:
Rho GTPase-activating protein 1Human Gene Id:
392Human Swiss Prot No:
Q07960Mouse Gene Id:
228359Mouse Swiss Prot No:
Q5FWK3Specificity:
ARHGAP1 Polyclonal Antibody detects endogenous levels of ARHGAP1 protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
ARHGAP1; CDC42GAP; RHOGAP1; Rho GTPase-activating protein 1; CDC42 GTPase-activating protein; GTPase-activating protein rhoOGAP; Rho-related small GTPase protein activator; Rho-type GTPase-activating protein 1; p50-RhoGAPMolecularWeight(Da):
50436Observed Band(KD):
50Function:
function:GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate.,similarity:Contains 1 CRAL-TRIO domain.,similarity:Contains 1 Rho-GAP domain.,subunit:Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with BNIPL.,tissue specificity:Ubiquitous.,Subcellular Location:
cytoplasm,cytosol,cell-cell adherens junction,integral component of membrane,perinuclear region of cytoplasm,extracellular exosome,Expression:
Fibrosarcoma,Pancreas,Platelet,Spleen,
- Western blot analysis of lysates from HeLa and HUVEC cells, using RHG1 Antibody. The lane on the right is blocked with the synthesized peptide.
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