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Akt2 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-70187

  • Amount:

    100ul

  • Molecular Weight:

    55769Da

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Akt2. AA range:432-481

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of 3T3 cells using Akt2 Polyclonal Antibody diluted at 1:500

  • Gene Name:

    AKT2

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

  • Background:

    This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq, Jul 2008],

  • Protein Name:

    RAC-beta serine/threonine-protein kinase

  • Human Gene Id:

    208

  • Human Swiss Prot No:

    P31751

  • Mouse Gene Id:

    11652

  • Mouse Swiss Prot No:

    Q60823

  • Rat Gene Id:

    25233

  • Rat Swiss Prot No:

    P47197

  • Specificity:

    Akt2 Polyclonal Antibody detects endogenous levels of Akt2 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    AKT2; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta

  • Observed Band(KD):

    56

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Alterations of AKT2 may contribute to the pathogenesis of ovarian carcinomas.,enzyme regulation:Two specific sites, one in the kinase domain (Thr-309) and the other in the C-terminal regulatory region (Ser-474), need to be phosphorylated for its full activation.,function:General protein kinase capable of phosphorylating several known proteins.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B.,tissue specificity:In all human cell types so far analyzed.,

  • Subcellular Location:

    Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Early endosome . Localizes within both nucleus and cytoplasm of proliferative primary myoblasts and mostly within the nucleus of differentiated primary myoblasts. By virtue of the N-terminal PH domain, is recruited to sites of the plasma membrane containing increased PI(3,4,5)P3 or PI(3,4)P2, cell membrane targeting is also facilitared by interaction with CLIP3. Colocalizes with WDFY2 in early endosomes (By similarity). .

  • Expression:

    Expressed in all cell types so far analyzed.

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Catalog Number

  • Amount:100ul
  • Price:225$

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Akt2 Polyclonal Antibody | EnoGene Biotech Co. Ltd