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AAK1 Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-70038

  • Amount:

    100ul

  • Molecular Weight:

    93578Da

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat;Monkey

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human AAK1. AA range:271-320

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using AAK1 Polyclonal Antibody

  • Gene Name:

    AAK1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

  • Background:

    Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008],

  • Protein Name:

    AP2-associated protein kinase 1

  • Human Gene Id:

    22848

  • Human Swiss Prot No:

    Q2M2I8

  • Mouse Gene Id:

    269774

  • Mouse Swiss Prot No:

    Q3UHJ0

  • Rat Swiss Prot No:

    P0C1X8

  • Specificity:

    AAK1 Polyclonal Antibody detects endogenous levels of AAK1 protein.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    AAK1; KIAA1048; AP2-associated protein kinase 1; Adaptor-associated kinase 1

  • Observed Band(KD):

    94

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Stimulated by clathrin.,function:Phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subcellular location:Active when found in clathrin-coated pits at the plasma membrane.,subunit:Interacts with alpha-adaptin and AP-2.,

  • Subcellular Location:

    Cell membrane ; Peripheral membrane protein . Membrane, clathrin-coated pit . Cell junction, synapse, presynapse . Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells. .

  • Expression:

    Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain.

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Catalog Number

  • Amount:100ul
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AAK1 Polyclonal Antibody | EnoGene Biotech Co. Ltd