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9G8 Polyclonal Antibody
Datasheet
Catalog Number:
E20-70028Amount:
100ulMolecular Weight:
27367DaApplications:
WB;IHC-p;IF(paraffin section);ELISAReactivity:
Human;MousePurification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human SFRS7. AA range:61-110Source:
Polyclonal, Rabbit,IgG
- Western blot analysis of the lysates from HT-29 cells using SFRS7 antibody.
Gene Name:
SRSF7Dilution:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.Background:
The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010],Protein Name:
Serine/arginine-rich splicing factor 7Human Gene Id:
6432Human Swiss Prot No:
Q16629Mouse Gene Id:
225027Mouse Swiss Prot No:
Q8BL97Specificity:
9G8 Polyclonal Antibody detects endogenous levels of 9G8 protein.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
SRSF7; SFRS7; Serine/arginine-rich splicing factor 7; Splicing factor 9G8; Splicing factor; arginine/serine-rich 7Observed Band(KD):
35Function:
alternative products:Isoforms, often lacking the RS domain and differentially expressed in fetal tissues, may be involved in modulation of 9G8 function,function:Required for pre-mRNA splicing. Can also modulate alternative splicing in vitro.,PTM:Extensively phosphorylated on serine residues in the RS domain.,similarity:Belongs to the splicing factor SR family.,similarity:Contains 1 CCHC-type zinc finger.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Found in large molecular weight complexes containing CCNL1 and the p110 isoforms of either CDC2L1 or CDC2L2. Interacts with CCNL2 and CPSF6.,tissue specificity:Brain, liver, kidney and lung.,Subcellular Location:
Nucleus . Cytoplasm .Expression:
Brain, liver, kidney and lung.
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