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MLK3 (phospho Ser674) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-61202

  • Amount:

    100ul

  • Molecular Weight:

    92688 Da

  • Applications:

    WB;IHC-p

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    Synthesized phospho-peptide around the phosphorylation site of human MLK3 (phospho Ser674)

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of extracts from NIH-3T3 cells, using Phospho-MLK3 Polyclonal Antibody.

  • Gene Name:

    MAP3K11

  • Dilution:

    WB 1:500-2000;IHC-p 1:50-300

  • Background:

    The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq, Jul 2008],

  • Protein Name:

    Mitogen-activated protein kinase kinase kinase 11

  • Human Gene Id:

    4296

  • Human Swiss Prot No:

    Q16584

  • Mouse Gene Id:

    26403

  • Mouse Swiss Prot No:

    Q80XI6

  • Rat Gene Id:

    309168

  • Rat Swiss Prot No:

    Q66HA1

  • Specificity:

    Phospho-MLK3 (S674) Polyclonal Antibody detects endogenous levels of MLK3 protein only when phosphorylated at S674.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    MAP3K11; MLK3; PTK1; SPRK; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Src-homology 3 domain-containing proline-rich kinase

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.,function:Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.,PTM:Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP

  • Subcellular Location:

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Location is cell cycle dependent.

  • Expression:

    Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.

  • Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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Catalog Number

  • Amount:100ul
  • Price:225$

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MLK3 (phospho Ser674) Polyclonal Antibody | EnoGene Biotech Co. Ltd