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Rb (phospho Thr821) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-61137

  • Amount:

    100ul

  • Swiss-Prot No.:

    P06400/P13405/P33568

  • Molecular Weight:

    106159

  • Applications:

    IHC-p,IF/ICC,ELISA

  • Reactivity:

    Human,Mouse,Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinitychromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human
    Retinoblastoma around the phosphorylation site of Thr821. AA range:791-840

  • Specificity/Sensitivity:

    Phospho-Rb (T821) Polyclonal Antibody detects endogenous levels of Rb
    protein only when phosphorylated at T821.

  • Storage/Stability:

    -20°C/1 year

  • Source:

    Rabbit

  • Gene Name:

    RB1

  • Dilution:

    Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000.
    ELISA: 1/40000. Not yet tested in other applications.

  • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non Phosphopeptide (Phospho-right), using Retinoblastoma (Phospho-Thr821) Antibody

  • Background:

    RB transcriptional corepressor 1(RB1) Homo sapiens The protein encoded by
    this gene is a negative regulator of the cell cycle and was the first tumor
    suppressor gene found. The encoded protein also stabilizes constitutive
    heterochromatin to maintain the overall chromatin structure. The active,
    hypophosphorylated form of the protein binds transcription factor E2F1. Defects
    in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer,
    and osteogenic sarcoma. [provided by RefSeq, Jul 2008]

  • Gene ID:

    5925/19645/24708

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Function:

    disease:Defects in RB1 are a cause of bladder cancer
    [MIM:109800].,disease:Defects in RB1 are a cause of osteogenic sarcoma
    [MIM:259500].,disease:Defects in RB1 are the cause of childhood cancer
    retinoblastoma (RB) [MIM:180200]. RB is a congenital malignant tumor that
    arises from the nuclear layers of the retina. It occurs in about 1:20'000 live births
    and represents about 2% of childhood malignancies. It is bilateral in about 30% of
    cases. Although most RB appear sporadically, about 20% are transmitted as an
    autosomal dominant trait with incomplete penetrance. The diagnosis is usually
    made before the age of 2 years when strabismus or a gray to yellow reflex from
    pupil ("cat eye") is investigated.,function:Key regulator of entry into cell division
    that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target
    genes. The underphosphorylated, active form of RB1 interacts

  • Immunofluorescence analysis of HeLa cells, using Retinoblastoma (Phospho-Thr821) Antibody. The picture on the right is blocked with the phospho peptide

  • Immunohistochemistry analysis of paraffin-embedded human testis, using Retinoblastoma (Phospho-Thr821) Antibody. The picture on the right is blocked with the phospho peptide.

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Rb (phospho Thr821) Polyclonal Antibody | EnoGene Biotech Co. Ltd