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SphK2 (phospho Thr614) Polyclonal Antibody
Datasheet
Catalog Number:
E20-61108Amount:
100ulPrice:
225$Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.Immunogen:
The antiserum was produced against synthesized peptide derived from human SPHK2 around the phosphorylation site of Thr614. AA range:580-629Source:
Polyclonal, RabbitGene Name:
SPHK2Dilution:
WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SPHK2 (Phospho-Thr614) Antibody. The picture on the right is blocked with the phospho peptide.
Background:
sphingosine kinase 2(SPHK2) Homo sapiens This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011],Protein Name:
Sphingosine kinase 2Human Gene Id:
56848Human Swiss Prot No:
Q9NRA0Specificity:
Phospho-SphK2 (T614) Polyclonal Antibody detects endogenous levels of SphK2 protein only when phosphorylated at T614.Formulation:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.Concentration:
1 mg/mlStorage Stability:
-20°C/1 yearOther Name:
SPHK2; Sphingosine kinase 2; SK 2; SPK 2MolecularWeight(Da):
69217Function:
alternative products:Experimental confirmation may be lacking for some isoforms,catalytic activity:ATP + sphinganine = ADP + sphinganine 1-phosphate.,catalytic activity:ATP + sphingosine = ADP + sphingosine 1-phosphate.,cofactor:Magnesium.,function:Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions.Subcellular Location:
lysosomal membrane,cytosol,membrane,Expression:
Brain,Carcinoma,Eye,Lymph,Whole embryo,
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using SPHK2 (Phospho-Thr614) Antibody
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