Bim (phospho Ser59) Polyclonal Antibody

Name: Bim (phospho Ser59) Polyclonal Antibody
Brand: EnoGene Biotech Co. Ltd
SKU: E20-61065
Price: 225 USD
Availability: InStock

E20-61065Cite ID: EN0049991Datasheet COA MSDS

ReactivityHuman;Mouse;Rat

ApplicationsIHC-p;IF(paraffin section);ELISA

SourcePolyclonal, Rabbit,IgG

$225/ 100ul

Quantity

Total: $225

Ship worldwide. Contact order@enogene.com for ordering and bulk pricing or contact your local distributor.

OverviewImages & Data (2)Documents

Product Information

Molecular Weight22171Da

Protein NameBcl-2-like protein 11

Human Gene Id10018

Human Swiss Prot NoO43521

Mouse Gene Id12125

Mouse Swiss Prot NoO54918

Rat Gene Id64547

Rat Swiss Prot NoO88498

SpecificityPhospho-Bim (S59) Polyclonal Antibody detects endogenous levels of Bim protein only when phosphorylated at S59.

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration1 mg/ml

Storage Stability-20°C/1 year

Other NameBCL2L11; BIM; Bcl-2-like protein 11; Bcl2-L-11; Bcl2-interacting mediator of cell death

Functiondomain:The BH3 motif is required for Bcl-2 binding and cytotoxicity.,function:Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.,similarity:Belongs to the Bcl-2 family.,subcellular location:Associated with intracytoplasmic membranes.,subunit:Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including MCL1, BCL2, BCL2L1 isoform Bcl-X(L), BCL2A1/BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK.,tissue specificity:Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lowe

Subcellular LocationEndomembrane system ; Peripheral membrane protein . Associated with intracytoplasmic membranes. .; [Isoform BimEL]: Mitochondrion. Translocates from microtubules to mitochondria on loss of cell adherence.; [Isoform BimL]: Mitochondrion.; [Isoform BimS]: Mitochondrion.; [Isoform Bim-alpha1]: Mitochondrion.

ExpressionIsoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are widely expressed with tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ImmunogenThe antiserum was produced against synthesized peptide derived from human BIM around the phosphorylation site of Ser59. AA range:31-80

Gene NameBCL2L11

DilutionImmunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

BackgroundThe protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2013],

📄Datasheet
Full product datasheet (PDF)✅Certificate of Analysis
Lot-specific COA (generated from product data)🧪MSDS
Material safety data sheet (generated)

Conjugated Antibody

This product is also available conjugated. Format: E20-61065-Conjugate. Price: $485 / Size: 100μl.

E20-61065-FITC$485 / 100μlE20-61065-Biotin$485 / 100μlE20-61065-HRP$485 / 100μlE20-61065-AP$485 / 100μlE20-61065-AF350$485 / 100μlE20-61065-AF405M$485 / 100μlE20-61065-AF488$485 / 100μlE20-61065-AF514$485 / 100μlE20-61065-AF532$485 / 100μlE20-61065-AF555$485 / 100μlE20-61065-AF568$485 / 100μlE20-61065-AF405S$485 / 100μlE20-61065-AF405L$485 / 100μlE20-61065-AF546$485 / 100μlE20-61065-AF594$485 / 100μlE20-61065-AF610$485 / 100μlE20-61065-AF635$485 / 100μlE20-61065-AF647$485 / 100μlE20-61065-AF680$485 / 100μlE20-61065-AF700$485 / 100μlE20-61065-AF750$485 / 100μlE20-61065-AF790$485 / 100μlE20-61065-APC$485 / 100μlE20-61065-PE$485 / 100μlE20-61065-Cy3$485 / 100μlE20-61065-Cy5$485 / 100μlE20-61065-Cy5.5$485 / 100μlE20-61065-Cy7$485 / 100μlE20-61065-PE-Cy5$485 / 100μl

Contact order@enogene.com to order conjugated formats. Magnetic Beads (1-30μm) conjugation is also available.