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Raf-1 (phospho Ser289) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-61058

  • Amount:

    100ul

  • Swiss-Prot No.:

    P04049/ Q99N57/ P11345

  • Molecular Weight:

    73052

  • Price:

    225$

  • Applications:

    IHC-p,IF(paraffin section),ELISA

  • Reactivity:

    Human,Mouse,Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinitychromatography using epitope-specific immunogen

  • Immunogen:

    Phosphopeptide (Phospho-left) and Non Phosphopeptide (Phospho-right), using C-RAF (PhosphoSer289) Antibody

  • Specificity/Sensitivity:

    Phospho-Raf-1 (S289) Polyclonal Antibody detects endogenous levels of Raf-1
    protein only when phosphorylated at S289.

  • Storage/Stability:

    -20°C/1 year

  • Source:

    Rabbit

  • Gene Name:

    RAF1

  • Dilution:

    Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other

  • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for

  • Background:

    Raf-1 proto-oncogene, serine/threonine kinase(RAF1) Homo sapiens This gene
    is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP
    kinase kinase kinase (MAP3K), which functions downstream of the Ras family of
    membrane associated GTPases to which it binds directly. Once activated, the
    cellular RAF1 protein can phosphorylate to activate the dual specificity protein
    kinases MEK1 and MEK2, which in turn phosphorylate to activate the
    serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are
    pleiotropic effectors of cell physiology and play an important role in the control of
    gene expression involved in the cell division cycle, apoptosis, cell differentiation
    and cell migration. Mutations in this gene are associated with Noonan syndrome 5
    and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008],

  • Gene ID:

    5894/110157/ 24703

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Binds 2zinc ions per subunit.,disease:Defects in RAF1 are the cause of LEOPARDsyndrome type 2 (LEOPARD syndrome-2) [MIM:611554]. LEOPARD syndrome isan autosomal dominant disorder allelic with Noonan syndrome. The acronymLEOPARD stands for lentigines, electrocardiographic conduction abnormalities,ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation ofgrowth, and deafness.,disease:Defects in RAF1 are the cause of Noonansyndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disordercharacterized by dysmorphic facial features, short stature, hypertelorism, cardiacanomalies, deafness, motor delay, and a bleeding diathesis. It is a geneticallyheterogeneous and relatively common syndrome, with an estimated incidence of1 in 1000-2500 live births.,function:Involved in the transducti

  • Immunohistochemistry analysis of paraffin-embedded human
    brain, using C-RAF (Phospho-Ser289) Antibody. The picture on
    the right is blocked with the phospho peptide.

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Catalog Number

  • Amount:100ul
  • Price:225$

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Raf-1 (phospho Ser289) Polyclonal Antibody | EnoGene Biotech Co. Ltd