You are here: EnoGene Home > Products > Cell Signal Transduction Research Antibodies > PERK (phospho Thr981) Polyclonal Antibody

PERK (phospho Thr981) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-61055

  • Amount:

    100ul

  • Molecular Weight:

    125147 Da

  • Applications:

    IF/ICC;WB;IHC-p;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human PEK/PERK around the phosphorylation site of Thr981. AA range:947-996

  • Source:

    Polyclonal, Rabbit,IgG

  • Wang, J., Xu, L., Xiang, Z. et al. Microcystin-LR ameliorates pulmonary fibrosis via modulating CD206+ M2-like macrophage polarization. Cell Death Dis 11, 136 (2020).

  • Gene Name:

    EIF2AK3

  • Dilution:

    IF: 1:50-200 WB 1:500-2000 ,Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

  • Background:

    The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015],

  • Protein Name:

    Eukaryotic translation initiation factor 2-alpha kinase 3

  • Human Gene Id:

    9451

  • Human Swiss Prot No:

    Q9NZJ5

  • Mouse Swiss Prot No:

    Q9Z2B5

  • Rat Gene Id:

    29702

  • Rat Swiss Prot No:

    Q9Z1Z1

  • Specificity:

    Phospho-PERK (T981) Polyclonal Antibody detects endogenous levels of PERK protein only when phosphorylated at T981.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    EIF2AK3; PEK; PERK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK

  • Observed Band(KD):

    130

  • Function:

    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.,domain:The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP.,enzyme regulation:Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5/BIP and oligomerization, resulting in transautophosphorylation and kinase act

  • Subcellular Location:

    Endoplasmic reticulum membrane; Single-pass type I membrane protein.

  • Expression:

    Ubiquitous. A high level expression is seen in secretory tissues.

  • Lei, Ting, et al. "Stress kinases, endoplasmic reticulum stress, and Alzheimer’s disease related markers in peripheral blood mononuclear cells from subjects with increased body weight." Scientific reports 6 (2016): 30890.

  • Liu, Daiqiang, et al. "Endoplasmic Reticulum Stress in Spinal Cord Contributes to the Development of Morphine Tolerance." Frontiers in molecular neuroscience 11 (2018): 72.

  • Western Blot analysis of various cells using Phospho-PERK Polyclonal Antibody diluted at 1:2000

Add to Cart

Related Products

PTEN (Phospho-Ser380) Antibody
PTEN (Phospho-Ser380) Antibody Datasheet  

  • Catalog Number:

    E011009-1

  • Amount:

    50μg/50μl

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    IHC: 1:50~1:100

  • Swiss-Prot No.:

    P60484

  • Price:

    145$
  • Add to Cart
PTEN (Phospho-Ser380) Antibody
PTEN (Phospho-Ser380) Antibody Datasheet  

  • Catalog Number:

    E011009-2

  • Amount:

    100μg/100μl

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    IHC: 1:50~1:100

  • Swiss-Prot No.:

    P60484

  • Price:

    225$
  • Add to Cart
NFκB-p65 (Phospho-Ser468) Antibody
NFκB-p65 (Phospho-Ser468) Antibody Datasheet  

  • Catalog Number:

    E011013-1

  • Amount:

    50μg/50μl

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 IF:1:100~1:200

  • Swiss-Prot No.:

    Q04206

  • Price:

    145$
  • Add to Cart
NFκB-p65 (Phospho-Ser468) Antibody
NFκB-p65 (Phospho-Ser468) Antibody Datasheet  

  • Catalog Number:

    E011013-2

  • Amount:

    100μg/100μl

  • Reactivity:

    Human, Mouse, Rat

  • Applications:

    WB: 1:500~1:1000 IF:1:100~1:200

  • Swiss-Prot No.:

    Q04206

  • Price:

    225$
  • Add to Cart
Rel (Phospho-Ser503) Antibody
Rel (Phospho-Ser503) Antibody Datasheet  

  • Catalog Number:

    E011020-1

  • Amount:

    50μg/50μl

  • Reactivity:

    Human

  • Applications:

    IF:1:100~1:200

  • Swiss-Prot No.:

    Q04864

  • Price:

    145$
  • Add to Cart

Catalog Number

  • Amount:100ul
  • Price:225$

Add to Cart

AbPromise

Contact us

PERK (phospho Thr981) Polyclonal Antibody | EnoGene Biotech Co. Ltd