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Emt (phospho Tyr512) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-61004

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human ITK around the phosphorylation site of Tyr512. AA range:478-527

  • Source:

    Polyclonal, Rabbit

  • Gene Name:

    ITK

  • Dilution:

    WB 1:500-2000 ,Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

  • Immunohistochemical analysis of paraffin-embedded Human testis. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

  • Background:

    IL2 inducible T-cell kinase(ITK) Homo sapiens This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008],

  • Protein Name:

    Tyrosine-protein kinase ITK/TSK

  • Human Gene Id:

    3702

  • Human Swiss Prot No:

    Q08881

  • Mouse Gene Id:

    16428

  • Mouse Swiss Prot No:

    Q03526

  • Specificity:

    Phospho-Emt (Y512) Polyclonal Antibody detects endogenous levels of Emt protein only when phosphorylated at Y512.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    ITK; EMT; LYK; Tyrosine-protein kinase ITK/TSK; Interleukin-2-inducible T-cell kinase; IL-2-inducible T-cell kinase; Kinase EMT; T-cell-specific kinase; Tyrosine-protein kinase Lyk

  • MolecularWeight(Da):

    71831

  • Observed Band(KD):

    72

  • Function:

    catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,cofactor:Binds 1 zinc ion per subunit.,function:Plays a role in T-cell proliferation and differentiation.,induction:By interleukin-2.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.,similarity:Contains 1 Btk-type zinc finger.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subcellular location:Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells.,tissue specificity:T-cell lines and natural killer cell lines.,

  • Subcellular Location:

    intracellular,cytosol,cell-cell junction,extrinsic component of cytoplasmic side of plasma membrane,

  • Expression:

    Human rectum tumor,Spleen,Thymus,

  • Western blot analysis of 293 using p-Emt (Y512) antibody.

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Catalog Number

  • Amount:100ul
  • Price:225$

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Emt (phospho Tyr512) Polyclonal Antibody | EnoGene Biotech Co. Ltd