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HNF4-α (phospho Ser313) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60951

  • Amount:

    100ul

  • Molecular Weight:

    52785 Da

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human HNF4 alpha around the phosphorylation site of Ser313. AA range:280-329

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-HNF4-α Polyclonal Antibody diluted at 1:1000

  • Gene Name:

    HNF4A

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

  • Background:

    The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012],

  • Protein Name:

    Hepatocyte nuclear factor 4-alpha

  • Human Gene Id:

    3172

  • Human Swiss Prot No:

    P41235

  • Mouse Gene Id:

    15378

  • Mouse Swiss Prot No:

    P49698

  • Rat Gene Id:

    25735

  • Rat Swiss Prot No:

    P22449

  • Specificity:

    Phospho-HNF4-α (S313) Polyclonal Antibody detects endogenous levels of HNF4-α protein only when phosphorylated at S313.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    HNF4A; HNF4; NR2A1; TCF14; Hepatocyte nuclear factor 4-alpha; HNF-4-alpha; Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4

  • Observed Band(KD):

    52

  • Function:

    alternative products:Additional isoforms seem to exist,disease:Defects in HNF4A are the cause of maturity onset diabetes of the young type 1 (MODY1) [MIM:125850]; also shortened MODY-1. MODY [MIM:606391] is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age) and a primary defect in insulin secretion. The clinical phenotype of MODY1 is characterized by severe insulin secretory defects, and by major hyperglycemia associated with microvascular complications.,function:Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.,miscellaneous:Binds fatty acids.,online information:Hepatocyte nuclear fac

  • Subcellular Location:

    Nucleus.

  • Expression:

    Kidney,Liver,

  • Enzyme-Linked Immunosorbent Assay for Immunogen Phosphopeptide and Non-Phosphopeptide, using HNF4 alpha Antibody

  • Immunohistochemistry analysis of paraffin-embedded human liver carcinoma, using HNF4 alpha Antibody. The picture on the right is blocked with the phospho peptide.

  • Western blot analysis of lysates from HUVEC cells treated with EGF 200ng/ml 30', using HNF4 alpha Antibody. The lane on the right is blocked with the phospho peptide.

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Catalog Number

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HNF4-α (phospho Ser313) Polyclonal Antibody | EnoGene Biotech Co. Ltd