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Net (phospho Ser357) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60920

  • Amount:

    100ul

  • Molecular Weight:

    44240Da

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Elk3 around the phosphorylation site of Ser357. AA range:323-372

  • Source:

    Polyclonal, Rabbit

  • Western Blot analysis of COLO cells using Phospho-Net (S357) Polyclonal Antibody

  • Gene Name:

    ELK3

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

  • Protein Name:

    ETS domain-containing protein Elk-3

  • Human Gene Id:

    2004

  • Human Swiss Prot No:

    P41970

  • Specificity:

    Phospho-Net (S357) Polyclonal Antibody detects endogenous levels of Net protein only when phosphorylated at S357.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    ELK3; NET; SAP2; ETS domain-containing protein Elk-3; ETS-related protein ERP; ETS-related protein NET; Serum response factor accessory protein 2; SAP-2; SRF accessory protein 2

  • Observed Band(KD):

    38

  • Background:

    ELK3, ETS transcription factor(ELK3) Homo sapiens This gene encodes a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015],

  • Function:

    function:May be a negative regulator of transcription, but can activate transcription when coexpressed with Ras, Src or Mos. Forms a ternary complex with the serum response factor and the ETS and SRF motifs of the Fos serum response element.,similarity:Belongs to the ETS family.,similarity:Contains 1 ETS DNA-binding domain.,subunit:Interacts with CTBP1.,

  • Subcellular Location:

    nucleus,nucleoplasm,mitochondrion,intracellular membrane-bounded organelle,

  • Expression:

    Brain,Epithelium,Placenta,

  • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Elk3 (Phospho-Ser357) Antibody

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Catalog Number

  • Amount:100ul
  • Price:225$

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Net (phospho Ser357) Polyclonal Antibody | EnoGene Biotech Co. Ltd