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Bad (phospho Ser134) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60910

  • Amount:

    100ul

  • Swiss-Prot No.:

    Q92934

  • Molecular Weight:

    18.3kd Observed Band 28kd

  • Applications:

    WB,IHC-p,IF/ICC,ELISA

  • Reactivity:

    Human,Mouse,Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human BAD around the phosphorylation site of Ser134. AA range:100-149

  • Specificity/Sensitivity:

    Phospho-Bad (S134) Polyclonal Antibody detects endogenous levels of Bad protein only when phosphorylated at S134.

  • Storage/Stability:

    -20°C/1 year

  • Source:

    Rabbit

  • Gene Name:

    BAD

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

  • Immunofluorescence analysis of HeLa cells, using BAD (Phospho-Ser134) Antibody. The picture on the right is blocked with the phospho peptide.

  • Background:

    BCL2 associated agonist of cell death(BAD) Homo sapiens The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008],

  • Gene ID:

    572

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

  • Function:

    domain:Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.,function:Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.,online information:Bcl 2-associated death promoter entry,PTM:Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site

  • Immunohistochemistry analysis of paraffin-embedded human brain, using BAD (Phospho-Ser134) Antibody. The picture on the right is blocked with the phospho peptide.

  • Western blot analysis of lysates from mouse liver, using BAD (Phospho-Ser134) Antibody. The lane on the right is blocked with the phospho peptide.

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Catalog Number

  • Amount:100ul
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Bad (phospho Ser134) Polyclonal Antibody | EnoGene Biotech Co. Ltd