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14-3-3 θ/τ (phospho Ser232) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60903

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human 14-3-3 thet/tau around the phosphorylation site of Ser232. AA range:196-245

  • Source:

    Polyclonal, Rabbit

  • Gene Name:

    YWHAQ

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

  • Immunofluorescence analysis of Hela cell. 1,14-3-3 θ/τ (phospho Ser232) Polyclonal Antibody(green) was diluted at 1:200(4° overnight). (red) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog:RS3608 was diluted at 1:1000(room temperature, 50min).

  • Background:

    tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta(YWHAQ) Homo sapiens This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008],

  • Protein Name:

    14-3-3 protein theta

  • Human Gene Id:

    5350

  • Human Swiss Prot No:

    P27348

  • Mouse Gene Id:

    22630

  • Mouse Swiss Prot No:

    P68254

  • Rat Gene Id:

    25577

  • Rat Swiss Prot No:

    P68255

  • Specificity:

    Phospho-14-3-3 θ/τ (S232) Polyclonal Antibody detects endogenous levels of 14-3-3 θ/τ protein only when phosphorylated at S232.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    YWHAQ; 14-3-3 protein theta; 14-3-3 protein T-cell; 14-3-3 protein tau; Protein HS1

  • MolecularWeight(Da):

    27764

  • Observed Band(KD):

    28

  • Function:

    function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.,similarity:Belongs to the 14-3-3 family.,subcellular location:In neurons, axonally transported to the nerve terminals.,subunit:Homodimer. Interacts with PCTK1 (By similarity). Interacts with SSH1. Interacts with CDKN1B ('Thr-198' phosphorylated form); the interaction translocates CDKN1B to the cytoplasm.,tissue specificity:Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in i

  • Subcellular Location:

    cytoplasm,mitochondrion,cytosol,focal adhesion,membrane,cytoplasmic vesicle membrane,protein complex,extracellular exosome,

  • Expression:

    B-cell lymphoma,Brain,Eye,H

  • Western blot analysis of 293T MOUSE-BRAIN using p-14-3-3 θ/τ (S232) antibody. Antibody was diluted at 1:500

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Catalog Number

  • Amount:100ul
  • Price:225$

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14-3-3 θ/τ (phospho Ser232) Polyclonal Antibody | EnoGene Biotech Co. Ltd