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TRAP220 (phospho Thr1457) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60890

  • Amount:

    100ul

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human PPAR-BP around the phosphorylation site of Thr1457. AA range:1423-1472

  • Source:

    Polyclonal, Rabbit

  • Gene Name:

    MED1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

  • Immunofluorescence analysis of HeLa cells, using PPAR-BP (Phospho-Thr1457) Antibody. The picture on the right is blocked with the phospho peptide.

  • Background:

    mediator complex subunit 1(MED1) Homo sapiens The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq, Jul 2008],

  • Protein Name:

    Mediator of RNA polymerase II transcription subunit 1

  • Human Gene Id:

    5469

  • Human Swiss Prot No:

    Q15648

  • Mouse Gene Id:

    19014

  • Mouse Swiss Prot No:

    Q925J9

  • Specificity:

    Phospho-TRAP220 (T1457) Polyclonal Antibody detects endogenous levels of TRAP220 protein only when phosphorylated at T1457.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    MED1; ARC205; CRSP1; CRSP200; DRIP205; DRIP230; PBP; PPARBP; PPARGBP; RB18A; TRAP220; TRIP2; Mediator of RNA polymerase II transcription subunit 1; Activator-recruited cofactor 205 kDa component; ARC205; Mediator complex subunit 1; Peroxiso

  • MolecularWeight(Da):

    168438

  • Observed Band(KD):

    168

  • Function:

    function:Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.,PTM:Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the Mediator complex subunit 1 family.,subcellular location:A subset of the protein may enter the nucleol

  • Subcellular Location:

    ubiquitin ligase complex,chromatin,nucleus,nucleoplasm,nucleolus,membrane,mediator complex,protein-DNA complex,

  • Expression:

    B-cell,Colon,Skin,

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Catalog Number

  • Amount:100ul
  • Price:225$

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TRAP220 (phospho Thr1457) Polyclonal Antibody | EnoGene Biotech Co. Ltd