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Caldesmon (phospho Ser789) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60874

  • Amount:

    100ul

  • Molecular Weight:

    high molecular weight (predominantly in smooth muscles) (120-150 KD) and a low molecular weight (60-90KD)

  • Applications:

    WB;IHC-p;IF/ICC;ELISA

  • Reactivity:

    Human;Mouse;Rat

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human Caldesmon around the phosphorylation site of Ser789. AA range:744-793

  • Source:

    Polyclonal, Rabbit,IgG

  • Western Blot analysis of various cells using Phospho-Caldesmon Polyclonal Antibody

  • Gene Name:

    CALD1

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

  • Background:

    This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008],

  • Protein Name:

    Caldesmon

  • Human Gene Id:

    800

  • Human Swiss Prot No:

    Q05682

  • Rat Gene Id:

    25687

  • Rat Swiss Prot No:

    Q62736

  • Specificity:

    Phospho-Caldesmon (S789) Polyclonal Antibody detects endogenous levels of Caldesmon protein only when phosphorylated at S789.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    CALD1; CAD; CDM; Caldesmon; CDM

  • Observed Band(KD):

    80

  • Function:

    domain:The N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form.,function:Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.,PTM:In non-muscle cells, phosphorylation by CDC2 during mit

  • Subcellular Location:

    Cytoplasm, cytoskeleton . Cytoplasm, myofibril . Cytoplasm, cytoskeleton, stress fiber . On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle). .

  • Expression:

    High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.

  • Western Blot analysis of 293 cells using Phospho-Caldesmon Polyclonal Antibody

  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Caldesmon Antibody. The picture on the right is blocked with the phospho peptide.

  • Western blot analysis of lysates from HeLa cells treated with EGF 200ng/ml 30', using Caldesmon Antibody. The lane on the right is blocked with the phospho peptide.

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Catalog Number

  • Amount:100ul
  • Price:225$

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Caldesmon (phospho Ser789) Polyclonal Antibody | EnoGene Biotech Co. Ltd