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EF-2 (phospho Thr56) Polyclonal Antibody
Datasheet   

  • Catalog Number:

    E20-60870

  • Amount:

    100ul

  • Molecular Weight:

    95207Da

  • Price:

    225$

  • Purification:

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

  • Immunogen:

    The antiserum was produced against synthesized peptide derived from human eEF2 around the phosphorylation site of Thr56. AA range:31-80

  • Source:

    Polyclonal, Rabbit

  • Western Blot analysis of SKOV3 cells using Phospho-EF-2 (T56) Polyclonal Antibody diluted at 1:2000

  • Gene Name:

    EEF2

  • Dilution:

    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

  • Protein Name:

    Elongation factor 2

  • Human Gene Id:

    1938

  • Human Swiss Prot No:

    P13639

  • Specificity:

    Phospho-EF-2 (T56) Polyclonal Antibody detects endogenous levels of EF-2 protein only when phosphorylated at T56.

  • Formulation:

    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration:

    1 mg/ml

  • Storage Stability:

    -20°C/1 year

  • Other Name:

    EEF2; EF2; Elongation factor 2; EF-2

  • Observed Band(KD):

    100

  • Background:

    eukaryotic translation elongation factor 2(EEF2) Homo sapiens This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008],

  • Function:

    function:This protein promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome.,PTM:Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A.,PTM:Phosphorylation by EF-2 kinase completely inactivates EF-2.,similarity:Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.,subunit:Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9.,

  • Subcellular Location:

    nucleus,cytoplasm,cytosol,plasma membrane,cell-cell adherens junction,membrane,aggresome,intracellular ribonucleoprotein complex,extracellular matrix,polysomal ribosome,membrane raft,extracellular exosome

  • Expression:

    Brain,Cajal-Retzius cell,Epithelium,Hepatocyte,Ovary,Periph

  • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using eEF2 (Phospho-Thr56) Antibody

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Catalog Number

  • Amount:100ul
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